Bovine IVD Monolayer Cultures

Product number

CDD-B-1700 Intervertebral disc NP culture

 

CDD-B-1701 Intervertebral disc AF culture

 

Features

· Flask or multi-well plate format: T-75 flask or 12, 24, 48 and 96-well

· Seeding density:100,000 cell/cm²

· Shipping media: 50:50 DMEM/Ham’s F12 supplemented with 10% FBS

· All cultures are guaranteed contamination-free

· Discounts apply for multiple plate orders

Tissue Procurement: Bovine intervertebral disc (IVD) tissue is collected from the tail of 18 month old cows. The intervertebral disc tissue is harvested into two pools, the nucleus pulposus (NP) and annulus fibrosus (AF); intermediate tissues are discarded.

 

Monolayer Culture Processing: Disc tissues are treated with a sequential enzyme cocktail to isolate cells from surrounding matrices. The cells are counted and transferred to a flask or multi-well plate in 50/50 DMEM/F12 media supplemented with 10% FBS. The cells are maintained in a humidified incubator at 37^oC and 5% CO₂ for 48 hours to promote cell adhesion and then shipped with cold packs for overnight delivery.

Monolayer cultures: cells seeded onto a T-75 flask and a 96-well plate

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