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Bovine Chondrocytes in Alginate Bead Cultures |
Product numberCDD-B-1200 Bovine chondrocytes in alginate beads
Features · Multi-well plate format: 12, 24, 48 and 96-well · Bulk format: Alginate beads shipped in a 50ml tube · Alginate beads can be distributed any number per well up to 96 beads per order. · Shipping media: 50:50 DMEM/Ham’s F12 supplemented with 10% FBS · All cultures are guaranteed contamination-free · Discounts apply for multiple plate orders |
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Tissue Procurement: Bovine cartilage is derived from the metacarpalphalangeal joint (“knuckle”) of 18 month old cows. Alternately cartilage can be harvested from the stifle joint (“knee”) or nasal septum for an additional procurement fee.
Alginate Bead Processing: Cartilage is digested with a sequence of enzymes to isolate primary chondrocytes. The cells are combined with alginate gel and added drop-wise into a calcium salt bath to initiate polymerization. Alginate beads are recovered and distributed into multi-well plates or into the desired format with 50/50 DMEM/F12 media supplemented with 10% FBS. Alginate bead cultures are shipped with ice packs for overnight delivery. |
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Cells encapsulated in alginate beads |
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ENGINEERING |
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A R T I C U L A R |
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Call 847-498-9564 |
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tissues, cultures and contract research |
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Questions regarding pricing or options? Contact us |