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Human Chondrocytes in Alginate Bead Cultures |
Product numberCDD-H-2200 Normal chondrocytes in alginate beads
CDD-H-2202 OA+ chondrocytes in alginate beads
CDD-H-2201 RA+ chondrocytes in alginate beads
Features · Multi-well plate format: 12, 24, 48 and 96-well · Bulk format: Alginate beads shipped in a 50ml tube · Alginate beads can be distributed any number per well up to 96 beads per order. · Shipping media: 50:50 DMEM/Ham’s F12 supplemented with 10% FBS · All cultures are guaranteed contamination-free · Discounts apply for multiple plate orders |
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Tissue Procurement: Human cartilage tissue is collected from cadaveric donors within 24 hours of death or as surgical specimens from a relevant procedure. The tissue is harvested from the surfaces of the knee joint from both clinically “normal” donors and from those diagnosed with rheumatoid and osteoarthritis. Proper consent and serological information is obtained on all specimens.
Alginate Bead Processing: Cartilage is digested with a sequence of enzymes to isolate primary chondrocytes. The cells are combined with alginate gel and added drop-wise into a calcium salt bath to initiate polymerization. Alginate beads are recovered and distributed into multi-well plates or into the desired format with 50/50 DMEM/F12 media supplemented with 10% FBS. Alginate bead cultures are shipped with ice packs for overnight delivery. |
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Human chondrocytes encapsulated in alginate beads (10X magnification) |
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ENGINEERING |
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Alginate beads for culture |
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A R T I C U L A R |
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Call 847-498-9564 |
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tissues, cultures and contract research |
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Questions regarding pricing or options? Contact us |